NF-H Recombinant Antibody [JF99-07] - Rat IgG1 (Chimeric) - BSA and Azide free (HA610394)
Catalog# HA610394
to NF-H
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of NF-H on different lysates with Rat anti-NF-H antibody (HA610394) at 1/2,000 dilution.
Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 112 kDa
Observed band size: 200 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610394) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse cerebellum tissue lysate (10 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 112 kDa
Observed band size: 200 kDa
Exposure time: 5 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610394) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.