Nectin-4 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH09-73] (HA751315)
Catalog# HA751315
Rabbit Monoclonal to Nectin-4
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Nectin-4 on different lysates with Rabbit anti-Nectin-4 antibody (HA751315) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: A549 cell lysate (negative) (15 µg/Lane)
Lane 3: MDA-MB-231 cell lysate (negative) (15 µg/Lane)
Lane 4: T-47D cell lysate (15 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 45-70 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751315) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Nectin-4 on different lysates with Rabbit anti-Nectin-4 antibody (HA751315) at 1/2,000 dilution.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: A549 cell lysate (negative) (15 µg/Lane)
Lane 3: MDA-MB-231 cell lysate (negative) (15 µg/Lane)
Lane 4: T-47D cell lysate (15 µg/Lane)
Predicted band size: 55 kDa
Observed band size: 45-70 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751315) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.