Nav1.6 / SCN8A Recombinant Rabbit Monoclonal Antibody [PSH22-36] (HA724294)
Catalog# HA724294
Rabbit Monoclonal to Nav1.6 / SCN8A
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Nav1.6 / SCN8A on different lysates with Rabbit anti-Nav1.6 / SCN8A antibody (HA724294) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse liver tissue lysate (negative) (no heat)
Lane 3: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 225 kDa
Observed band size: 260 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724294) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Negative control : Liver (PMID : 11532991)
Western blot analysis of Nav1.6 / SCN8A on different lysates with Rabbit anti-Nav1.6 / SCN8A antibody (HA724294) at 1/2,000 dilution.
Lane 1: Mouse brain tissue lysate (no heat)
Lane 2: Mouse liver tissue lysate (negative) (no heat)
Lane 3: Rat brain tissue lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 225 kDa
Observed band size: 260 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA724294) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Negative control : Liver (PMID : 11532991)