MYH14 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH20-41] (HA751757)
Catalog# HA751757
Rabbit Monoclonal to MYH14
Tested for
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WB
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IHC-P
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IF-Cell
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FC
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IP
Reacts with
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Human
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Mouse
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Rat
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Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MYH14 on different lysates with Rabbit anti-MYH14 antibody (HA751757) at 1/5,000 dilution.
Lane 1: COLO205 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Lane 6: Mouse colon tissue lysate (40 µg/Lane)
Lane 7: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 228 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751757) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: COLO205 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: HCT 116 cell lysate (20 µg/Lane)
Lane 4: HEK-293 cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Lane 6: Mouse colon tissue lysate (40 µg/Lane)
Lane 7: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 228 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751757) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.