MUC5B Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-64] (HA751905)
Catalog# HA751905
Rabbit Monoclonal to MUC5B
Tested for
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IHC-P
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IF-Tissue
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Application: Immunohistochemistry (IHC-P)
Species: Human
Tissue: Seromucous gland
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751905, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.
Species: Human
Tissue: Seromucous gland
Sample: Paraffin-embedded section
Antigen retrieval: Heat-mediated, Tris-EDTA buffer (pH 9.0), 20 minutes at 95°C.
Wash buffer: 1× TBST
Endogenous peroxidase blocking: 3% H₂O₂, 10 minutes at room temperature.
Blocking: 1% BSA + 10% normal goat serum, 10 minutes at room temperature.
Primary antibody: HA751905, 1/1,000, 1 hour at room temperature.
Secondary antibody: HA1119, 20 minutes at room temperature.