MTHFD2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-73] (HA751951)
Catalog# HA751951
Rabbit Monoclonal to MTHFD2
Tested for
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WB
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IF-Cell
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IHC-P
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FC
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IP
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MTHFD2 on different lysates with Rabbit anti-MTHFD2 antibody (HA751951) at 1/5,000 dilution.
Lane 1: HEK-293 (Human embryonic kidney cell)cell lysate
Lane 2: MDA-MB-231 (Human breast cancer cell)cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cell)cell lysate
Lane 4: 786-0 (Human renal clear cell adenocarcinoma cell)cell lysate
Lane 5: MEF (Mouse embryonic fibroblast)cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 8 seconds ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751951, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 38 kDa
Observed band size: 35 kDa
Lane 1: HEK-293 (Human embryonic kidney cell)cell lysate
Lane 2: MDA-MB-231 (Human breast cancer cell)cell lysate
Lane 3: HeLa (Human cervical adenocarcinoma cell)cell lysate
Lane 4: 786-0 (Human renal clear cell adenocarcinoma cell)cell lysate
Lane 5: MEF (Mouse embryonic fibroblast)cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 8 seconds ; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751951, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 38 kDa
Observed band size: 35 kDa