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Mouse/Rat IGF1 enzyme-linked immunoassay kit（one step）(EMY0024)

Price: Regular price $629.00

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Catalog# EMY0024

Assay type Sandwich ELISA

Standard curve range 1.23~300 ng/ml

Sensitivity 0.10 pg/ml (50 μl)；0.37 pg/ml (10 μl)；

Reacts with

  • Mouse

  • Rat

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.00540.00600.0057
1.230.01100.01050.01080.0051
3.070.01930.01970.01950.0138
7.680.04360.04840.04600.0403
19.200.12560.12960.12760.1219
48.000.42300.40380.41340.4077
120.001.38701.33901.36301.3573
300.003.39503.32403.35953.3538

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)6.428.9120.66.730.4127.4
Standard Deviation0.21.15.90.31.35.5
Coefficient of Variation(%)3.73.74.93.84.24.3

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum3064.64-353.03100179.59

Sensitivity

0.10 pg/ml (50 μl)；0.37 pg/ml (10 μl)；

Standard curve range

1.23~300 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse/rat IGF-I/IGF-1 into health mouse/rat serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 119% with an overall mean recovery of 99%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse/Rat IGF1 enzyme-linked immunoassay kit（one step）(EMY0024)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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