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Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit(one step) (EMY0045)

Price: Regular price $629.00

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Catalog# EMY0045

Assay type Sandwich ELISA

Standard curve range 0.27~200 ng/ml

Sensitivity 0.01 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.00590.00630.0061
0.270.01960.02040.02000.0139
0.820.04740.05300.05020.0441
2.470.14120.15200.14660.1405
7.410.40250.42080.41170.4056
22.221.11401.21701.16551.1594
66.672.86602.87302.86952.8634
200.004.19544.35024.27284.2667

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)5.426.474.14.021.479.3
Standard Deviation0.42.14.30.20.44.1
Coefficient of Variation(%)6.77.85.84.82.05.1

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum30146.01-320.89100223.31

Sensitivity

0.01 pg/ml (10 μl)

Standard curve range

0.27~200 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of Mouse Pro-collagen I alpha 1/COL1A1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 86% to 119% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse Pro-collagen I alpha 1/COL1A1 enzyme-linked immunoassay kit(one step) (EMY0045)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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