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Mouse IL-33 enzyme-linked immunoassay kit (EM0033)

Price: Regular price $499.00

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Catalog# EM0033

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 0.33 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01760.01740.0175
6.860.02250.02300.02280.0053
20.580.03420.03500.03460.0171
61.730.06750.07100.06930.0518
185.190.17370.17670.17520.1577
555.560.50580.54630.52610.5086
1666.671.30301.45301.37801.3605
5000.003.05703.14603.10153.0840

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)117.2525.01698.489.6465.71468.5
Standard Deviation5.833.775.02.822.169,2
Coefficient of Variation(%)5.06.44.43.24.74.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-2.55530.53

Sensitivity

0.33 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-33 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 84% to 107% with an overall mean recovery of 94%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-33 enzyme-linked immunoassay kit (EM0033)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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