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Mouse IL-17E enzyme-linked immunoassay kit(EM0028)

Price: Regular price $499.00

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Catalog# EM0028

Assay type Sandwich ELISA

Standard curve range 468.75~30000 pg/ml

Sensitivity 12.33 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.03620.03240.0343
468.750.07330.07880.07610.0540
937.500.12510.12180.12350.1014
1875.000.22390.22520.22460.2025
3750.000.43440.43570.43510.4130
7500.000.87700.83570.85640.8343
15000.001.84201.84101.84151.8195
30000.003.71103.83303.77203.7500

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)573.62641.97988.6582.22756.58431.0
Standard Deviation38.4115.8476.738.0133.9514,2
Coefficient of Variation(%)6.74.46.06.54.96.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-427.6075199.53

Sensitivity

12.33 pg/ml (10 μl)

Standard curve range

468.75~30000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-17E/IL-25 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 99% to 118% with an overall mean recovery of 109%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-17E enzyme-linked immunoassay kit(EM0028)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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