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Mouse IL-1 beta Super Sensitivity enzyme-linked immunoassay kit (EMSS0029)

Price: Regular price $619.00

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Catalog# EMSS0029

Assay type Sandwich ELISA

Standard curve range 1.56~100 pg/ml

Sensitivity 0.12 pg/ml (50 μl)；0.36 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.12800.13600.1320
1.560.15300.17240.16270.0307
3.130.19020.20520.19770.0657
6.250.27920.28090.28010.1481
12.500.43700.44540.44120.3092
25.000.76250.72190.74220.6102
50.001.30501.31901.31201.1800
100.002.50702.46602.48652.3545

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)1.89.729.21.89.729.4
Standard Deviation0.0300.0050.0020.0320.0050.002
Coefficient of Variation(%)5.54.74.55.74.84.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum300.18-3.931001.47

Sensitivity

0.12 pg/ml (50 μl)；0.36 pg/ml (10 μl)

Standard curve range

1.56~100 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IL-1 beta into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 111% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IL-1 beta Super Sensitivity enzyme-linked immunoassay kit (EMSS0029)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P10749

PROTEIN NAME

IL-1 beta

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