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Mouse IFN alpha-1 enzyme-linked immunoassay kit (EM0049)

Price: Regular price $499.00

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Catalog# EM0049

Assay type Sandwich ELISA

Standard curve range 78.13~5000 pg/ml

Sensitivity 2.65 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02550.02880.0272
78.130.10230.11110.10670.0796
156.250.18860.18890.18880.1616
312.500.37050.36040.36550.3383
625.000.74180.72270.73230.7051
1250.001.40511.45271.42891.4018
2500.002.73032.58472.65752.6304
5000.004.07424.18144.12784.1007

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)87.84491.571544.4088.73480.461517.12
Standard Deviation4.9918.6866.802.5315.3757.77
Coefficient of Variation(%)5.73.84.32.83.23.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-26.3066.713.29

Sensitivity

2.65 pg/ml (10 μl)

Standard curve range

78.13~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse IFN alpha-1 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 93% to 98% with an overall mean recovery of 96%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse IFN alpha-1 enzyme-linked immunoassay kit (EM0049)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P01572

PROTEIN NAME

IFN alpha-1

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