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Mouse FGF-21 enzyme-linked immunoassay kit (EM0053)

Price: Regular price $499.00

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Catalog# EM0053

Assay type Sandwich ELISA

Standard curve range 8.19~2000 pg/ml

Sensitivity 2.46 pg/ml（10 μl）

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01710.01750.0173
8.190.02560.02690.02630.0090
20.480.03740.04060.03900.0217
51.200.07270.07850.07560.0583
128.000.17050.17810.17430.1570
320.000.38880.43560.41220.3949
800.000.97141.08401.02771.0104
2000.002.87002.52602.69802.6807

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)55.4247.5704.844.6210.5599.7
Standard Deviation2.712.940.71.39.713.3
Coefficient of Variation(%)4.85.25.82.94.62.2

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3012.29-921.03100196.81

Sensitivity

2.46 pg/ml（10 μl）

Standard curve range

8.19~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse FGF-21 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 93% to 120% with an overall mean recovery of 108%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse FGF-21 enzyme-linked immunoassay kit (EM0053)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

Q9JJN1

PROTEIN NAME

FGF-21

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