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Mouse CD86 enzyme-linked immunoassay kit (EM0046)

Price: Regular price $499.00

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Catalog# EM0046

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 0.28 pg/ml (10 μl)

Reacts with

  • Mouse

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00930.00870.0090
6.860.03620.03310.03470.0257
20.580.09490.09950.09720.0882
61.730.29520.31400.30460.2956
185.190.79600.73310.76460.7556
555.561.96202.01401.98801.9790
1666.673.25603.19503.22553.2165
5000.003.89203.90403.89803.8890

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)182.88551.761826.16159.79469.111545.97
Standard Deviation7.5519.78110.288.7526.9989.35
Coefficient of Variation(%)4.13.66.05.55.85.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30119.78-359.50100265.68

Sensitivity

0.28 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of mouse CD86 into health mouse serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 97% to 104% with an overall mean recovery of 99%.

Specifications

Product Type

ELISA Kit

Product Name

Mouse CD86 enzyme-linked immunoassay kit (EM0046)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P42082

PROTEIN NAME

CD86

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