Mouse CD45R/B220 Recombinant Rat Monoclonal Antibody [PSH10-80] (HA601385)
Catalog# HA601385
Rat Monoclonal to Mouse CD45R
Tested for
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IHC-P
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FC
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IF-Tissue
Reacts with
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Rat anti-Mouse CD45R/B220 antibody (HA601385) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601385) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601385) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.