Human/Mouse MMP-2 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Capture) [PSH22-04] (HA725379)
Catalog# HA725379
Rabbit Monoclonal to MMP-2
Tested for
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ELISA(Cap)
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Sandwich ELISA analysis of Human MMP-2 matched pair antibodies
Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04]
Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725379) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-2 protein (HA210660) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725380, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 9 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture: HA725379, Human/Mouse MMP-2 Rabbit mAb [PSH22-04]
Detector: HA725380, Human/Mouse MMP-2 Rabbit mAb [PSH22-05]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725379) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human MMP-2 protein (HA210660) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725380, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 9 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.