MLD Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH02-54] (HA750817)
Catalog# HA750817
Rabbit Monoclonal to MLD
Tested for
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WB
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IHC-P
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IF-Tissue
Reacts with
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Human
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Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MLD on different lysates with Rabbit anti-MLD antibody (HA750817) at 1/1,000 dilution.
Lane 1: A549 cell lysate
Lane 2: 293T cell lysate
Lane 3: HeLa cell lysate
Lane 4: PC-3M cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 34 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate
Lane 2: 293T cell lysate
Lane 3: HeLa cell lysate
Lane 4: PC-3M cell lysate
Lane 5: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 38 kDa
Observed band size: 34 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750817) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.