This panel covers the major functional cell populations and status markers in the human immune microenvironment, including: Lymphoid cells: detecting regulatory T cells (FOXP3), CD4+ T cells, CD8+ T cells, B cells (CD20, CD21, CD38), NK cells (CD57), etc., to analyze the composition of adaptive immune responses. Myeloid cells: detecting neutrophils (MPO, CD14), dendritic cells (CD11c), macrophages (CD68), M2 macrophages (CD163), mast cells (Tryptase), to elucidate innate immune components and immunomodulatory functions. Mesenchymal cells: detecting vascular endothelial cells (CD31), fibroblast activation protein (FAP), and myofibroblasts (aSMA), for the study of the matrix and vascular microenvironment. Epithelial/tumor markers Epithelial/Tumor Labeling: PanCK is used to label tumor cells and epithelial cells to aid in determining tumor boundaries and cell origin.
Proliferation: Ki67 is used to label cell proliferation activity, assessing cell activity and treatment response.
Immune Checkpoints: PD-1 and PD-L1 are used for analyzing immunosuppressive mechanisms and predicting immunotherapy response.
This panel is suitable for panoramic analysis of the tumor immune microenvironment, enabling multi-dimensional detection of lymphocytes, myeloid cells, stroma, tumor cells, and their functional status in a single experiment. It is particularly suitable for predicting immunotherapy efficacy, discovering biomarkers, and studying mechanisms.
