MDC1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-24] (HA751892)
Catalog# HA751892
Rabbit Monoclonal to MDC1
Tested for
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WB
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IF-Cell
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of MDC1 on different lysates with Rabbit anti-MDC1 antibody (HA751892) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cells) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751892, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 227 kDa
Observed band size: 227/260-320 kDa
Lane 1: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 2: HEK-293 (Human embryonic kidney cells) cell lysate
Lane 3: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 4: NIH/3T3 (Mouse fibroblasts) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 2 minutes; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751892, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 227 kDa
Observed band size: 227/260-320 kDa