MCU Mouse Monoclonal Antibody [A2E11] (EM1901-85)
Catalog# EM1901-85
Mouse Monoclonal to MCU
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WB
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IHC-P
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IF-Cell
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Human
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Mouse
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Rat
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Monkey
Lane 1: HCT 116 cell lysate (20 µg/Lane)
Lane 2: PANC-1 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: HeLa cell lysate (20 µg/Lane)
Lane 5: COS-1 cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C2C12 cell lysate (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: PC-12 cell lysate (20 µg/Lane)
Lane 10: Mouse colon tissue lysate (20 µg/Lane)
Lane 11: Mouse testis tissue lysate (20 µg/Lane)
Lane 12: Rat colon tissue lysate (20 µg/Lane)
Lane 13: Rat heart tissue lysate (20 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 33 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-85) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
MCU Mouse Monoclonal Antibody [A2E11] (EM1901-85)
Immunogen
Recombinant protein within human mcu aa 51-325 / 351.
Host
Mouse
Positive Control
HCT 116 cell lysate, PANC-1 cell lysate, A549 cell lysate, HeLa cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, Mouse colon tissue lysate, Mouse testis tissue lysate, Rat colon tissue lysate, Rat heart tissue lysate, human colon tissue, human colon cancer tissue, HCT 116, NIH/3T3, C6.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A2E11
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:50
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IF-Cell
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1:50
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 40 kDa
Isotype
IgM
TARGET
UNIPROT #
PROTEIN NAME
MCU
SYNONYMS
C109A_HUMAN antibody;C10orf42 antibody;Calcium uniporter protein mitochondrial antibody;Ccdc109a antibody;Coiled-coil domain-containing protein 109A antibody;HsMCU antibody;Mitochondrial Calcium Uniporter antibody
SEQUENCE SIMILARITIES
Belongs to the MCU (TC 1.A.77) family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation by CaMK2 in heart leads to increased MCU current. The regulation of MCU by CaMK2 is however subject to discussion: another group was unable to reproduce these results. Phosphorylated on tyrosines by PTK2B/PYK2, promoting oligomerization.
SUBCELLULAR LOCATION
Mitochondrion.
FUNCTION
Mitochondrial inner membrane calcium uniporter that mediates calcium uptake into mitochondria. Constitutes the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex). Activity is regulated by MICU1 and MICU2. At low Ca(2+) levels MCU activity is down-regulated by MICU1 and MICU2; at higher Ca(2+) levels MICU1 increases MCU activity. Mitochondrial calcium homeostasis plays key roles in cellular physiology and regulates cell bioenergetics, cytoplasmic calcium signals and activation of cell death pathways. Involved in buffering the amplitude of systolic calcium rises in cardiomyocytes. While dispensable for baseline homeostatic cardiac function, acts as a key regulator of short-term mitochondrial calcium loading underlying a 'fight-or-flight' response during acute stress: acts by mediating a rapid increase of mitochondrial calcium in pacemaker cells. participates in mitochondrial permeability transition during ischemia-reperfusion injury. Regulates glucose-dependent insulin secretion in pancreatic beta-cells by regulating mitochondrial calcium uptake. Mitochondrial calcium uptake in skeletal muscle cells is involved in muscle size in adults. Regulates synaptic vesicle endocytosis kinetics in central nerve terminal. Involved in antigen processing and presentation.
CITATIONS
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Ruthenium red attenuates acute pancreatitis by inhibiting MCU and improving mitochondrial function
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WB
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Mouse
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Citation
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