LYPD3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PS00-59] (HA750615)
Catalog# HA750615
Rabbit Monoclonal to LYPD3
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of LYPD3 on different lysates with Rabbit anti-LYPD3 antibody (HA750615) at 1/1,000 dilution.
Lane 1: SK-Br-3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 90 kDa
Exposure time: 1 minute 17 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750615) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: SK-Br-3 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 90 kDa
Exposure time: 1 minute 17 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750615) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.