Lamin A + Lamin C Recombinant Mouse Monoclonal Antibody - BSA and Azide free [A6F5-R] (HA610149)
Catalog# HA610149
Mouse Monoclonal to Lamin A + Lamin C
Tested for
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WB
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IF-Cell
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Knock-Out or Knock-Down validated
Western blot analysis of Lamin A + Lamin C on different lysates with Mouse anti-Lamin A + Lamin C antibody (HA610149) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74/65 kDa
Observed band size: 74/65 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610149) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: THP-1 cell lysate
Lane 4: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74/65 kDa
Observed band size: 74/65 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610149) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.