L1CAM Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JM11-05] (HA751842)
Catalog# HA751842
Rabbit Monoclonal to L1CAM
Tested for
-
WB
-
IHC-P
-
IF-Tissue
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (HA751842) at 1/1,000 dilution.
Lane 1: A375 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (low expression) (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (20 µg/Lane)
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751842) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of L1CAM on different lysates with Rabbit anti-L1CAM antibody (HA751842) at 1/1,000 dilution.
Lane 1: A375 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (low expression) (20 µg/Lane)
Lane 3: HeLa cell lysate (20 µg/Lane)
Lane 4: Human brain tissue lysate (20 µg/Lane)
Predicted band size: 140 kDa
Observed band size: 250 kDa
Exposure time: 10 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751842) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.