KMT1E / SETDB1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH20-39] (HA751755)
Catalog# HA751755
Rabbit Monoclonal to KMT1E / SETDB1
Tested for
-
WB
-
IHC-P
-
IP
Reacts with
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Human
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Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of KMT1E / SETDB1 on different lysates with Rabbit anti-KMT1E / SETDB1 antibody (HA751755) at 1/5,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: T-47D cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lane 5: K-562 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: F9 cell lysate
Lane 9: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 143 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751755) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Jurkat cell lysate
Lane 2: T-47D cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lane 5: K-562 cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: F9 cell lysate
Lane 9: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 143 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751755) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.