Klotho Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [JM93-76] (HA750445)
Catalog# HA750445
Rabbit Monoclonal to Klotho
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Klotho on human kidney tissue lysates with Rabbit anti-Klotho antibody (HA750445) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 116 kDa
Observed band size: 61 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750445) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 116 kDa
Observed band size: 61 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA750445) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.