iPS Cell Reprogramming Antibody Kit (HAK21032)
Catalog# HAK21032
Reacts with
-
Human
All lanes: Western blot analysis of Oct4 with anti-Oct4 antibody (ET1612-20) at 1/500 dilution.
Lane 1: Wild-type NCCIT whole cell lysate.
Lane 2: Oct4 knockout NCCIT whole cell lysate.
ET1612-20 was shown to specifically react with Oct4 in Wild-type NCCIT cells. No band was observed when Oct4 knockout sample was tested. Wild-type and Oct4 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Oct4 antibody (ET1612-20, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: Wild-type NCCIT whole cell lysate.
Lane 2: Oct4 knockout NCCIT whole cell lysate.
ET1612-20 was shown to specifically react with Oct4 in Wild-type NCCIT cells. No band was observed when Oct4 knockout sample was tested. Wild-type and Oct4 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-Oct4 antibody (ET1612-20, 1/500) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.