Involucrin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH23-99] (HA751921)
Catalog# HA751921
Rabbit Monoclonal to Involucrin
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Involucrin on different lysates with Rabbit anti-Involucrin antibody (HA751921) at 1/5,000 dilution.
Lane 1: HaCaT (Human immortalized keratinocytes) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 4: SK-BR-3 (Human breast cancer cells) cell lysate
Lane 5: NCI-H441 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 secondss; ECL: K1801
Negative expression of Involucrin protein in HeLa cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751921, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 68 kDa
Observed band size: 100~150 kDa
Western blot analysis of Involucrin on different lysates with Rabbit anti-Involucrin antibody (HA751921) at 1/5,000 dilution.
Lane 1: HaCaT (Human immortalized keratinocytes) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: A431 (Human epidermoid carcinoma skin squamous cells) cell lysate
Lane 4: SK-BR-3 (Human breast cancer cells) cell lysate
Lane 5: NCI-H441 (Human lung adenocarcinoma cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 7 secondss; ECL: K1801
Negative expression of Involucrin protein in HeLa cell is consistent with the predicted expression pattern
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751921, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 68 kDa
Observed band size: 100~150 kDa