intestinal alkaline phosphatase Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH17-82] (HA751641)
Catalog# HA751641
Rabbit Monoclonal to intestinal alkaline phosphatase
Tested for
-
WB
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of intestinal alkaline phosphatase on different lysates with Rabbit anti-intestinal alkaline phosphatase antibody (HA751641) at 1/5,000 dilution.
Lane 1: Saos-2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse small intestine tissue lysate (30 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 80 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751641) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Saos-2 cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (negative) (20 µg/Lane)
Lane 3: Mouse small intestine tissue lysate (30 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 80 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751641) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.