Immature Neuron Marker Antibody Sampler Kit (HAK21046)
Catalog# HAK21046
Reacts with
-
Human
Western blot analysis of Beta III Tubulin on different lysates with Mouse anti-Beta III Tubulin antibody (M0805-8) at 1/2,000 dilution.
Lane 1: SH-SY5Y cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: A-172 cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 11 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0805-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: SH-SY5Y cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: A-172 cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat brain tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 11 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M0805-8) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.