IL-6R Recombinant Rabbit Monoclonal Antibody [PSH22-78] (HA724323)
Catalog# HA724323
Rabbit Monoclonal to IL-6R
Tested for
-
WB
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA724323) at 1/2,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: U-937 (Human acute monocytic leukemia cells) cell lysate
Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate
Lane 5: U266 (Human multiple myeloma cell) cell lysate
Lane 6: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Negative control: K-562 and Daudi (PMID : 8300137).
Negative expression of IL-6R protein in HeLa cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724323, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 52 kDa
Observed band size: 70-100 kDa
Western blot analysis of IL-6R on different lysates with Rabbit anti-IL-6R antibody (HA724323) at 1/2,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cells) cell lysate
Lane 2: HeLa (Human cervical adenocarcinoma cells) cell lysate
Lane 3: U-937 (Human acute monocytic leukemia cells) cell lysate
Lane 4: Daudi (Human Burkitt's lymphoma cells) cell lysate
Lane 5: U266 (Human multiple myeloma cell) cell lysate
Lane 6: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 3 minutes; ECL: K1801
Negative control: K-562 and Daudi (PMID : 8300137).
Negative expression of IL-6R protein in HeLa cell is consistent with the predicted expression pattern.
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA724323, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 52 kDa
Observed band size: 70-100 kDa