IL-33 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH17-37] (HA751625)
Catalog# HA751625
Rabbit Monoclonal to IL-33
Tested for
-
WB
-
IHC-P
Reacts with
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE),Cell treatment (CT)
Western blot analysis of IL-33 on different lysates with Rabbit anti-IL-33 antibody (HA751625) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate (negative)
Lane 5: Rat lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751625) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-33 on different lysates with Rabbit anti-IL-33 antibody (HA751625) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: RAW264.7 treated with 100nM TPA overnight then 100ng/mL LPS for 7 hours add 1μg/mL BFA for last 3 hours cell lysate
Lane 3: Mouse lung tissue lysate
Lane 4: Mouse brain tissue lysate (negative)
Lane 5: Rat lung tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 30 kDa
Observed band size: 35 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751625) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.