IL-18 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH12-04] (HA751511)
Catalog# HA751511
Rabbit Monoclonal to IL-18
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (HA751511) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: J774A.1 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (negative) (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751511) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of IL-18 on different lysates with Rabbit anti-IL-18 antibody (HA751511) at 1/5,000 dilution.
Lane 1: RAW264.7 cell lysate (20 µg/Lane)
Lane 2: J774A.1 cell lysate (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (negative) (20 µg/Lane)
Lane 4: Mouse spleen tissue lysate (40 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751511) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.