Human IL-17F Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Detector) [PSH18-69] (HA725316)
Catalog# HA725316
Rabbit Monoclonal to IL-17F
Tested for
-
ELISA(Det)
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Sandwich ELISA analysis of human IL17F matched pair antibodies
Capture: HA725315, Human IL-17F Rabbit mAb [PSH18-68]
Detector: HA725316, Human IL-17F Rabbit mAb [PSH18-69]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725315) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-17F protein (HA210716) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725316, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 12 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture: HA725315, Human IL-17F Rabbit mAb [PSH18-68]
Detector: HA725316, Human IL-17F Rabbit mAb [PSH18-69]
Elisa assay was performed by coating wells of a 96-well plate with 50 µl per well of capture antibody (HA725315) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-17F protein (HA210716) starting from 20,000 pg/ml to 0 pg/ml and detect antibody (HA725316, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 50 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 12 minutes at 37℃ in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.