IL-10RB Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH19-60] (HA751723)
Catalog# HA751723
Rabbit Monoclonal to IL-10RB
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IL-10RB on different lysates with Rabbit anti-IL-10RB antibody (HA751723) at 1/5,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 cell lysate (no heat)
Lane 3: HDLM-2 cell lysate
Lane 4: HUVEC cell lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 55-75 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751723) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 cell lysate (no heat)
Lane 3: HDLM-2 cell lysate
Lane 4: HUVEC cell lysate
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37 kDa
Observed band size: 55-75 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751723) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.