IGFBP6 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH20-44] (HA751760)
Catalog# HA751760
Rabbit Monoclonal to IGFBP6
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of IGFBP6 on different lysates with Rabbit anti-IGFBP6 antibody (HA751760) at 1/5,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: HeLa cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 35 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751760) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: U-87 MG cell lysate
Lane 2: HeLa cell lysate
Lane 3: SH-SY5Y cell lysate
Lane 4: NCI-H1299 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 35 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751760) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.