Mouse/Rat IGFBP3 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Detector) [PSH22-90] (HA724335)
Catalog# HA724335
Rabbit Monoclonal to IGFBP3
Tested for
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ELISA(Det)
Reacts with
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Sandwich ELISA analysis of Mouse IGFBP3 matched pair antibodies
Capture: HA724334, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-89]
Detector: HA724335, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-90]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724334) diluted in carbonate/bicarbonate buffer, at a concentMouseion of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGFBP3 protein (HA211243) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724335, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstMousee for 10 minutes at room tempeMouseure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Capture: HA724334, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-89]
Detector: HA724335, Mouse/Rat IGFBP3 Rabbit mAb [PSH22-90]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA724334) diluted in carbonate/bicarbonate buffer, at a concentMouseion of 5 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.2% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Mouse IGFBP3 protein (HA211243) starting from 10,000 pg/ml to 0 pg/ml and detect antibody (HA724335, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB SubstMousee for 10 minutes at room tempeMouseure in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.