iFluor™ 800cwse Conjugated Goat anti-mouse IgG polyclonal Antibody(For Odyssey system) (HA1149)
Catalog# HA1149
Goat Polyclonal to Mouse IgG (H+L)
Tested for
-
WB
Reacts with
-
Mouse
Conjugated to iFluor™ 800cwse
Western blot analysis of β-tubulin and Histone H3 on different lysates at 1/20,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 50/15 kDa
Observed band size: 50/15 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (β-tubulin / Histone H3) at 1/20,000 dilution was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - iFluor™ 800cwse Secondary Antibody (HA1149) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Neuro-2a cell lysate
Lane 6: PC-12 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 50/15 kDa
Observed band size: 50/15 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (β-tubulin / Histone H3) at 1/20,000 dilution was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - iFluor™ 800cwse Secondary Antibody (HA1149) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.