iFluor™ 800cwse Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system) (HA1152)
Catalog# HA1152
Goat Polyclonal to Goat Anti-Rabbit IgG (H+L)
Tested for
-
WB
Reacts with
-
Rabbit
Conjugated to iFluor™ 800cwse
Western blot analysis of GAPDH and HSP90 on different lysates at 1/20,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: PC-12 cell lysate
Lane 3: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37/90 kDa
Observed band size: 37/90 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 800cwse Secondary Antibody (HA1152) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.
Lane 1: HeLa cell lysate
Lane 2: PC-12 cell lysate
Lane 3: HepG2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37/90 kDa
Observed band size: 37/90 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 800cwse Secondary Antibody (HA1152) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.