iFluor™ 750 Conjugated Goat Anti-Rabbit IgG (H+L) Goat Polyclonal Antibody (HA1134)
Catalog# HA1134
Goat Polyclonal to Goat anti-rabbit IgG
Tested for
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IF-Cell
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IF-Tissue
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FC
Reacts with
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Rabbit
Conjugated to iFluor™ 750
Flow cytometric analysis of HeLa cells labeling GAPDH.
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-4, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1134) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Cells were fixed and permeabilized. Then stained with the primary antibody (ET1601-4, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 750 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1134) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).