iFluor™ 680se Conjugated Goat anti-rabbit IgG polyclonal Antibody(For Odyssey system) (HA1150)
Catalog# HA1150
Goat Polyclonal to Goat Anti-Rabbit IgG (H+L)
Tested for
-
WB
Reacts with
-
Rabbit
Conjugated to iFluor™ 680se
Western blot analysis of GAPDH and HSP90 on different lysates at 1/20,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 25μM MG-132 for 4 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37/90 kDa
Observed band size: 37/90 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 680se Secondary Antibody (HA1150) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 10μM MG-132 for 6 hours cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: NIH/3T3 treated with 10μM MG-132 for 8 hours cell lysate
Lane 5: C6 cell lysate
Lane 6: C6 treated with 25μM MG-132 for 4 hours cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 37/90 kDa
Observed band size: 37/90 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (GAPDH / HSP90) at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - iFluor™ 680se Secondary Antibody (HA1150) at 1/5,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.