iFluor™ 680se Conjugated Goat anti-mouse IgG polyclonal Antibody(For Odyssey system) (HA1130)
Catalog# HA1130
Goat Polyclonal to Goat Anti-Mouse IgG (H+L)
Tested for
-
WB
Reacts with
-
Mouse
Conjugated to iFluor™ 680se
Western blot analysis of β-actin on different lysates with Mouse anti-β-actin antibody at 1/10,000 dilution.
Lane 1: EA.hy926 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: bEnd.3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at 1/10,000 dilution was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - iFluor™ 680se Secondary Antibody (HA1130) at 1/10,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.
Lane 1: EA.hy926 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: bEnd.3 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21002) at 1/10,000 dilution was used in TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - iFluor™ 680se Secondary Antibody (HA1130) at 1/10,000 dilution was used for 1 hour at room temperature and then imaged using the Licor Odyssey CLx.