iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44] (HA720173F)
Catalog# HA720173F
Rabbit Monoclonal to SATB2
Tested for
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IF-Tissue
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FC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 647
Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling SATB2 (HA720173F).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody SATB2 (HA720173F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody SATB2 (HA720173F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.