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Iba1 Recombinant Antibody - Rat IgG1 (Chimeric) (IRS158RT)

Price: Regular price $278.00

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Catalog# IRS158RT

to Iba1

Tested for

  • mIHC

Reacts with

  • Mouse

Recombinant production enables lot-to-lot consistency and is animal-cruelty-free

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Specifications

Product Type

Recombinant Chimeric Antibody primary

Product Name

Iba1 Recombinant Antibody - Rat IgG1 (Chimeric) (IRS158RT)

Immunogen

Synthetic peptide within n-terminal human iba1.

Positive Control

Mouse brain tissue.

Conjugation

Unconjugated

APPLICATION DILUTION

  • mIHC

  • 1:100

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4C. Store at +4C short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20C long term.

Storage Buffer

1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950.

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 17 kDa

Isotype

IgG1

TARGET

UNIPROT #

O70200

PROTEIN NAME

Iba1

SYNONYMS

AIF 1 antibody;AIF-1 antibody;Aif1 antibody;AIF1 protein antibody;AIF1_HUMAN antibody;Allograft inflammatory factor 1 antibody;Allograft inflammatory factor 1 splice variant G antibody;allograft inflammatory factor-1 splice variant Hara-1 antibody;balloon angioplasty responsive transcription antibody;BART 1 antibody;G1 antibody;G1 putative splice variant of allograft inflamatory factor 1 antibody;IBA 1 antibody;IBA1 antibody;interferon gamma responsive transcript antibody;Interferon responsive transcript 1 antibody;interferon responsive transcript factor 1 antibody;Ionized calcium binding adapter molecule 1 antibody;Ionized calcium-binding adapter molecule 1 antibody;ionized calcium-binding adapter molecule antibody;IRT 1 antibody;IRT1 antibody;Microglia response factor antibody;MRF1 antibody;Protein g1 antibody

SUBCELLULAR LOCATION

Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.

FUNCTION

Ionized calcium-binding adapter molecule 1 (Iba1), also known as allograft inflammatory factor-1 (AIF-1), is a 147 amino acid cytoplasmic, calcium-binding protein that is thought to play a role in macrophage activation and function. Iba1, containing two EF domains, is induced by cytokines and interferons. In an unstimulated state, Iba1 colocalizes with actin, and upon stimulation, translocates to lamellipodia. It is also a marker of human microglia and is expressed by macrophages in injured skeletal muscle. The gene encoding Iba1 maps to chromosome 6p21.33 and resides in the tumor necrosis factor (TNF) cluster of genes located in the region represented by the human major histocompatibility complex (MHC).

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