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Human tPA enzyme-linked immunoassay kit(one step) (EHY0158)

Price: Regular price $629.00

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Catalog# EHY0158

Assay type Sandwich ELISA

Standard curve range 0.14~100 ng/ml

Sensitivity 0.03 ng/ml (50 μl)；0.10 ng/ml (10 μl)；

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.00580.00640.0061
0.140.01070.00960.01020.0041
0.410.01950.01810.01880.0127
1.230.04570.04500.04540.0393
3.700.14060.12610.13340.1273
11.110.41910.37760.39840.3923
33.331.19001.17001.18001.1739
100.002.83702.87802.85752.8514

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)1.89.029.21.89.431.7
Standard Deviation0.10.41.50.10.41.8
Coefficient of Variation(%)5.74.65.15.23.95.6

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum303.24-20.651009.25

Sensitivity

0.03 ng/ml (50 μl)；0.10 ng/ml (10 μl)；

Standard curve range

0.14~100 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human tPA into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 97% to 115% with an overall mean recovery of 104%.

Specifications

Product Type

ELISA Kit

Product Name

Human tPA enzyme-linked immunoassay kit(one step) (EHY0158)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P00750

PROTEIN NAME

PAI-1/Serpin E1

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