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Human S100A8 & S100A9 Heterodimer, N-Strep II, C-His Tag Protein (HA211445)

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Catalog# HA211445

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  • Human

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Product Details

Product Type

Protein

Product Name

Human S100A8 & S100A9 Heterodimer, N-Strep II, C-His Tag Protein (HA211445)

Predicted molecular mass

12.7 kDa (S100A8) and 14.5 kDa (S100A9)

Protein construction description

A DNA sequence encoding the human S100A8 (P05109) (Met1-Glu93) was fused with a strep II tag at the N-terminus, constructed the plasmid 1; A DNA sequence encoding the human S100A9 (P06702) (Met1-Pro114) was fused with a his tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the S100A8 & S100A9 heterodimer was purified.

Accession

P05109 & P06702

Protein construction

Twin Strep | S100A8 (1-93) & S100A9 (1-114) | His

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

Calprotectin, a member of the widespread calcium-binding S-100 protein family, is present in remarkably high concentration in the cytoplasm of human neutrophils. The calprotectin molecule is composed of light (MRP8) and heavy (MRP14) subunits. Although not secreted from intact neutrophils, calprotectin release from dead and dying neutrophils creates high concentrations of the protein in inflammatory or abscess fluids and in the intestinal tract lumen of patients with inflammatory bowel disease.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃ It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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