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Human RAGE enzyme-linked immunoassay kit(one step) (EHY0222)

Price: Regular price $629.00

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Catalog# EHY0222

Assay type Sandwich ELISA

Standard curve range 156.25~10000 pg/ml

Sensitivity 21.39 pg/ml (50 μl)；65.37 pg/ml (10 μl)；

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00680.00600.0064
156.250.01780.01850.01820.0118
312.500.03460.03840.03650.0301
625.000.08840.08970.08910.0827
1250.000.24290.24990.24640.2400
2500.000.68100.69990.69050.6841
5000.001.84601.89801.87201.8656
10000.003.81203.81603.81403.8076

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)201.91011.43082.6218.51013.63171.6
Standard Deviation9.648.7122.77.233.4105.4
Coefficient of Variation(%)4.84.84.03.33.33.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30720.92-9110.871002431.31

Sensitivity

21.39 pg/ml (50 μl)；65.37 pg/ml (10 μl)；

Standard curve range

156.25~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human RAGE into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 122% with an overall mean recovery of 102%.

Specifications

Product Type

ELISA Kit

Product Name

Human RAGE enzyme-linked immunoassay kit(one step) (EHY0222)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

Q15109

PROTEIN NAME

RAGE

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