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Human PHGDH, N-Twin Strep, C-His, Flag Tag Protein (HA210678)

Price: Regular price $379.00

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Catalog# HA210678

Reacts with

  • Human

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Product Details

Product Type

Protein

Product Name

Human PHGDH, N-Twin Strep, C-His, Flag Tag Protein (HA210678)

Predicted molecular mass

63.3 kD

Protein construction description

A DNA sequence encoding the human PHGDH protein (O43175) (Met 1-Phe 533) was expressed with a Twin strep tag at the N-terminus and both His, Flag tag at the C-terminus.

Accession

O43175

Protein construction

Twin Strep | PHGDH (1-533) | His | Flag

Source

E.coli

Bio Activity

Testing in progress.

Purity

>95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

This gene encodes the enzyme which is involved in the early steps of L-serine synthesis in animal cells. L-serine is required for D-serine and other amino acid synthesis. The enzyme requires NAD/NADH as a cofactor and forms homotetramers for activity. Mutations in this gene have been found in a family with congenital microcephaly, psychomotor retardation and other symptoms. Multiple alternatively spliced transcript variants have been found, however the full-length nature of most are not known. Catalyzes the reversible oxidation of 3-phospho-D-glycerate to 3-phosphonooxypyruvate, the first step of the phosphorylated L-serine biosynthesis pathway. Also catalyzes the reversible oxidation of 2-hydroxyglutarate to 2-oxoglutarate and the reversible oxidation of (S)-malate to oxaloacetate.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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