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Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step) (EHY0160)

Price: Regular price $629.00

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Catalog# EHY0160

Assay type Sandwich ELISA

Standard curve range 0.05~40 ng/ml

Sensitivity 2.18 pg/ml (50 μl)；40.00 pg/ml (10 μl)

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.00730.00710.0072
0.050.01280.01280.01280.0056
0.160.02400.02230.02320.0160
0.490.05780.05460.05620.0490
1.480.16590.15230.15910.1519
4.440.51470.49480.50480.4976
13.331.58701.56301.57501.5678
40.003.82603.86303.84453.8373

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)0.94.112.30.83.812.2
Standard Deviation0.00.10.40.00.20.3
Coefficient of Variation(%)3.12.83.04.74.22.7

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum3098.03-475.14100264.19

Sensitivity

2.18 pg/ml (50 μl)；40.00 pg/ml (10 μl)

Standard curve range

0.05~40 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human PAI-1/Serpin E1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 101% with an overall mean recovery of 94%.

Specifications

Product Type

ELISA Kit

Product Name

Human PAI-1/Serpin E1 enzyme-linked immunoassay kit(one step) (EHY0160)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P05121

PROTEIN NAME

PAI-1/Serpin E1

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