Human Osteopontin Recombinant Rabbit Monoclonal Antibody - BSA and Azide free (Capture) [PSH08-58] (HA723004)
Catalog# HA723004
Rabbit Monoclonal to Human Osteopontin
Tested for
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ELISA(Cap)
Reacts with
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Human
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Cynomolgus monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Sandwich ELISA analysis of Human Osteopontin matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723004) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Osteopontin protein (HA210993) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723005, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA723004) diluted in carbonate/bicarbonate buffer, at a concentration of 5ug/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant Human Osteopontin protein (HA210993) starting from 5,000 pg/ml to 0 pg/ml and detect antibody (HA723005, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.