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Human MMP-13 enzyme-linked immunoassay kit (EH0135)

Price: Regular price $499.00

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Catalog# EH0135

Assay type Sandwich ELISA

Standard curve range 78.13~5000 pg/ml

Sensitivity 5.09 pg/ml (50 μl)；10.80 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.07920.08430.0818
78.130.14800.15240.15020.0685
156.250.23630.21710.22670.1450
312.500.35850.36770.36310.2814
625.000.60690.60870.60780.5261
1250.001.10201.09401.09801.0163
2500.001.89001.96601.92801.8463
5000.003.39303.35603.37453.2928

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)56.9372.01283.477.7432.41499.5
Standard Deviation3.322.374.82.813.238.0
Coefficient of Variation(%)5.86.05.83.63.12.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum305.75-44.2010021.14

Sensitivity

5.09 pg/ml (50 μl)；10.80 pg/ml (10 μl)

Standard curve range

78.13~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human MMP-13 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 92% to 117% with an overall mean recovery of 106%.

Specifications

Product Type

ELISA Kit

Product Name

Human MMP-13 enzyme-linked immunoassay kit (EH0135)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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